| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Nashville United Soccer Academy - 01 NUSA B07 Maroon |
0534-011B5U-0049 | Adam Harris |
| A2 | Tennessee Soccer Club TSC 2007 Boys Nike |
0522-011B5U-0080 | Jordan Salisbury |
| A3 | Emerald Force Soccer Club - 01 Emerald Force 07 Atletico |
0214-011B5U-4580 | Adelmo Santos |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2007 Boys Showcase |
0522-012B5U-0133 | Shane Barrett |
| A5 | Lobos Rush (Collierville Soccer Association) 07 Lobos Rush Premier |
0603-011B5U-0046 | David McAlister |
| A6 | Tennessee United Soccer Club TUSC 2007 Boys NPL |
0424-011B5U-0016 | Eduardo Carvacho |
| A7 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 07B Red |
0324-021B5U-0025 | Christopher Zeller |
| A8 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B15U Black |
0521-011B5U-0006 | B Ortega |