| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Legends 07 Premier |
0606-011B5U-0205 | Justin Rose |
| A2 | All-In FC TN All-In FC TN 07 Boys |
0443-01SLB5U-0074 | Stone Boston |
| A3 | Germantown Legends Germantown Legends 07 Black |
0606-012B5U-0216 | Kirk Dunaway |
| A4 | Emerald Force Soccer Club - 01 Emerald Force 07 Rangers |
0214-012B5U-4581 | Paul Bankston |
| A5 | Tennessee Soccer Club TSC Williamson 2007 Boys Showcase |
0522-012B5U-0079 | Matthew Ellis |
| A6 | Nashville Now Soccer 365 - 01 Nashville Now Soccer 365 - 0448 - B15U |
0448-012B5U-0001 | Jomo Cromwell |
| A7 | Chattanooga FC Academy Chattanooga FC Academy - 2007B Navy |
0316-022B5U-7822 | Dalton Healy |
| A8 | Tri-Cities United Soccer Club Tri-Cities United 07 Boys Premier |
0106-012B5U-0006 | Matthew Quigley |