| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Kings Hammer MSC 06 Strikers Showcase |
0515-012B6U-0126 | Raul Lopez |
| A2 | Jackson Soccer Club JSC 2006 Boys |
0609-012B6U-0012 | Malang Jarju |
| A3 | Clarksville Soccer Club Clarksville SC - B2006 Maroon |
0422-012B6U-0011 | Scott Dollar |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 06B Premier |
0206-012B6U-0042 | Kevin Cardona |
| A5 | Tennessee United Soccer Club TUSC 2006 Boys Red |
0424-012B6U-0019 | Miguel Arriaga |
| A6 | Lobos Rush (Collierville Soccer Association) 06 Lobos Rush Elite |
0603-012B6U-0038 | Carlos Rivera |
| A7 | Pateadores Soccer Club - Nashville Barca Academy 2006B Blue |
0500-01D2B6U-0011 | M Harispuru - DO NOT USE |
| A8 | Chattanooga FC Academy CSM Forge 16U |
0316-022B6U-7861 | Matthew Jelley |