| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 06 Lobos Rush Premier |
0603-011B6U-0028 | Carlos Rivera |
| A2 | Tennessee Soccer Club TSC 2006 Boys Nike |
0522-011B6U-0085 | Tyler Kahn |
| A3 | Tri-Cities United Soccer Club Tri-Cities United 06 Boys Premier |
0106-011B6U-0007 | Nicolas Boegemann |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2006 Boys Showcase |
0522-011B6U-2065 | Jason Finley |
| A5 | Tennessee United Soccer Club TUSC 2006 Boys NPL |
0424-011B6U-0018 | Nate Sego |
| A6 | Chattanooga Red Wolves Academy Chattanooga Red Wolves 06B Red |
0324-021B6U-0030 | Kuda Nhiwatiwa |
| A7 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B6U-0010 | Sunebari Ndam |
| A8 | Stones River Futbol Club - 01 Stones River Futbol Club - 01 - B16U Black |
0521-011B6U-0007 | Jun Taulbee |