| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Legends 04 Black NL |
0606-011B8U-0208 | Carey Talley |
| A2 | Nashville United Soccer Academy - 01 NUSA B04 Maroon |
0534-011B8U-0012 | Tommy Cochran |
| A3 | Tennessee Soccer Club TSC Nashville 2004 Boys Showcase |
0522-011B8U-0092 | Anthony Torres |
| A4 | Memphis Futbol Club / Memphis Soccer Education Memphis FC 04 Boys |
0624-011B8U-0008 | William Pryor |
| A5 | Tennessee Soccer Club TSC Murfreesboro 2004 Boys Showcase |
0522-011B8U-2009 | Jorge Suarez |
| A6 | Lobos Rush (Collierville Soccer Association) 04 Lobos Rush Premier |
0603-011B8U-0024 | David McAlister |
| A7 | Tennessee Soccer Club TSC Sumner 2004 Boys Showcase |
0522-011B8U-2061 | Daniel Sidebottom |
| A8 | Emerald Force Soccer Club - 01 Emerald Force 04 Celtic |
0214-011B8U-0069 | Kelvin Scott |