| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC Academy - 2003B Navy |
0316-022B9U-7817 | Darren Combs |
| A2 | Wilson United Soccer Club Wilson United - B19U |
0406-012B9U-0009 | Terrence Mashoro |
| A3 | Greeneville Soccer Association Greeneville Soccer Association - B19U |
0102-012B9U-7804 | Jonathan Houghton |
| A4 | Bartlett Soccer Club Bartlett Soccer Club - B2003/04 |
0626-012B9U-0231 | Walter Aeschliman |
| A5 | FC Alliance ChallengeTeams FC Alliance C - B18U |
0204-022B9U-0002 | Alexander Rigatti |
| A6 | Kononia Soccer Club - 01 Kononia Soccer PWYS -01 - B19U |
0611-012B9U-1976 | Bernardo Ferreira |
| A7 | Lobos Rush (Collierville Soccer Association) 03/04 Lobos Rush Showcase |
0603-012B9U-0050 | Isaac Johnson |
| A8 | Knoxville FC (Crush) Knoxville FC Crush 04/03 B Premier |
0206-012B9U-0010 | Fabio Suarez |