| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Clarksville Soccer Club Clarksville SC - G2010 Maroon |
0422-01AG2U-0018 | Kelley Guth |
| A2 | Gallatin Rangers GSC Lady Rangers 10 |
0408-02AG2U-0051 | Jordan Sharp |
| A3 | Arlington Soccer Academy - 01 Arlington Soc - G12U |
0627-01AG2U-0067 | Ryan Paule |
| A4 | Tennessee Soccer Club TSC Williamson 2010 Girls Academy Premier |
0522-01AG2U-2067 | Lance Mayes |
| A5 | Clarksville Soccer Club Clarksville SC - G2011 Maroon |
0422-01AG1U-0003 | Ty Burdine |
| A6 | Columbia Soccer Association - 01 Columbia Lady Arsenal 2010 |
0504-015G2U-0182 | Dustin Butler |
| A7 | Knoxville FC (Crush) Knoxville FC Crush 2010G Dortmund |
0206-01CG2U-5556 | Paige Huffaker |
| A8 | Tennessee United Soccer Club TUSC 2010 Girls Blue |
0424-01AG2U-0041 | Ray Trotter |